Variations can be observed in pollinator assemblages of exotic species in response to changes in environment or to variables that did not receive much attention. This paper is the first approximation of the abundance of pollinator species associated with Eucalyptus nitens in a Clonal seed-bank plantation (CSBP) of the farm "Rucamanqui", Huepil, Biobío Region in Chile. Between October and November 2010, we examined the variation of pollinator's entomofauna of E. nitens. Environmental factors, such as temperature and the number of open flowers per plant, were registered to determine the level of dependence on the frequency of visits. The most abundant insects visiting E. nitens belonged to the orders Coleoptera, Hymenoptera, Diptera and Lepidoptera. However, the proportion of individuals in each group of flower visitors varied considerably among the sampling months: Hymenoptera (203), Coleoptera (21) in October and Coleoptera (280), Hymenoptera (57) in November. For Hymenoptera, environmental temperature was significant compared to frequency of visitors; whereas for Coleoptera, the number of open flowers per plant has been significant for insect activity. Results show that the visit frequency of pollinators in E. nitens is highly variable within the sampling period and temperature and density of open flowers per plant can produce variations within the group of pollinators.